Ampha X30

Fast and accurate determination of cell count, viability and metabolic activity – without staining, labeling or incubation.

Technical Specifications

• Detection principle: Impedance Flow Cytometry (label-free)
• Cell size range: 1 – 250 µm
• Sample volume: Typically 100 µL
• Measured parameters: Cell count, viability, cell size, metabolic status
• Dimensions (W × D × H): Approx. 40 × 30 × 30 cm

The Ampha X30 is a universal benchtop instrument for label-free single-cell analysis based on Impedance Flow Cytometry (IFC). It enables simultaneous measurement of cell count, size, viability, and metabolic status for all types of cells – from bacteria to mammalian cells – in real time and without the need for dyes, markers, or calibration.

Key Features

• Label-free and calibration-free
  No fluorescent stains, markers, or apoptosis kits required. No incubation or calibration steps necessary.
• Comprehensive cell characterization
  Simultaneous measurement of cell count and viability for all cell types from 1 µm to 50 µm – including insights into cell health and metabolism.
• Reliable even in complex media
  Electrical detection is unaffected by turbidity, opacity, or autofluorescence, allowing measurements in complex matrices such as milk, polymer emulsions, and microalgae cultures.
• Fast and cost-efficient
  Minimal sample preparation, measurement within seconds, and low operating and investment costs.

Typical Applications

• Bacterial counting and viability
  Rapid and accurate quantification of live and dead bacteria – no need for incubation or plating.
• Yeast fermentation and bioprocess monitoring
  Track cell growth and vitality in real time to optimize fermentation processes.
• Metabolic status monitoring
  Detect changes in membrane integrity to assess nutrient depletion or stress conditions in cell cultures.
• Protein expression in insect cells
  Monitor infection kinetics and determine the optimal harvest time during Baculovirus processes.
• Cytotoxicity and apoptosis testing
  Identify apoptotic cells even when nanoparticles interfere with fluorescent markers.
• Contamination detection
  Distinguish and quantify multiple cell populations in mixed samples – no labeling required.

Technology

The Ampha X30 applies Impedance Flow Cytometry (IFC), analyzing individual cells through changes in electrical impedance as they pass through a microfluidic chip.
This approach provides quantitative, label-free information on each cell’s size, membrane integrity, and metabolic activity — independent of optical transparency.

Benefits for Your Laboratory

• Suitable for research and industrial laboratories
• Provides rapid, real-time insight for process optimization and control
• Works with bacteria, yeast, algae, mammalian, and plant cells
• Advanced software for visualization and analysis of single-cell data