Detection and quantitative determination of pemoline (Stimul®) in urine
Pemoline is abused in sports for doping purposes and is therefore on the IOC (Int. Olimp. Committee) list of banned drugs.
A cost effective screening method is described allowing to analyse a large number of samples at the same time. If the chromatographic derivatisation reaction indicates the presence of pemoline, the re3sult is verified by spectra comparison.
Densitometric quantification is performed by absorbance at 220 nm with the underivatized fraction. Determination limit is o.1 mg/L, reliable detection limit
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