Fat determination in Animal feed samples using Twisselmann extraction
The determination of fat in food is a routine procedure used in quality assurance and for labelling. Below, a simple and easy procedure for fat determination in animal feed samples, according to COMMISSION REGULATION (EC) No 152/2009 is introduced. The sample is hydrolysed with hydrochloric acid, followed by a Twisselmann extraction with the Extraction Unit E-816 ECE (Economic Continuous Extraction). The determined fat contents correspond well to the labelled values.
The reference value was determined with Soxhlet extraction following Weibull-Stoldt.
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